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| Product Name: |
Human Betacellulin(BTC) ELISA Kit
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| Supply Ability: |
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| Related proudcts |
btc, |
| Specifications |
E0001Hu |
| Price Term: |
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| Port of loading: |
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1. Can¡¯t ***ect the sample which contain NaN3, because NaN3 inhibits HRP active.
2. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can¡¯t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
3. serum- coagulation at room temperature 10-20 mins£¬centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
4.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation ofHydrothorax and cerebrospinal fluid Reference to it.
6.cell culture supernatant-***ect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,***ect the composition of cells, Dilut cell suspension with PBS£¨PH7.2-7.4£©, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
7.Tissue samples- After cutting samples, check the weight,add PBS£¨PH7.2-7.4£©, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS£¨PH7.4£©, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant. |
| Company: |
Shanghai Crystal day Biotech Co., Ltd.
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| Contact: |
Ms. Yu Chen |
| Address: |
Central Plains Road, Yangpu District, Shanghai, Lane 60, Zhongyuan Building, Building 1, Room 1306 |
| Postcode: |
200438 |
| Tel: |
86-21-55493350 |
| Fax: |
86-21-65578917 |
| E-mail: |
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